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Fig. 3 | Nanoscale Research Letters

Fig. 3

From: SPIO Enhance the Cross-Presentation and Migration of DCs and Anionic SPIO Influence the Nanoadjuvant Effects Related to Interleukin-1β

Fig. 3

Migration and location of EGFP-DCs after labelling with SPIO. a Laser scanning confocal microscopy of EGFP-DCs labeled with 25 μg/mL SPIO nanoparticles after 12 h of incubation. b, c Fluorescence intensity of the SPIO-labeled EGFP-DCs after 12 h. In vitro optical imaging of drainage lymph nodes after the back transfusion of SPIO-labeled EGFP-DCs at different days. TNF-α was first injected into the mouse foot pad in advance to promote the migration of EGFP-DCs. de In vitro optical imaging and signal intensity analysis of lymph nodes on different days after injection of EGFP-DCs with or without SPIO. f EGFP positive cells of draining lymph nodes were detected by laser confocal microscopy

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