Fig. 4From: Amine-Aldehyde Chemical Conjugation on a Potassium Hydroxide-Treated Polystyrene ELISA Surface for Nanosensing an HIV-p24 AntigenOptimization of the incubation period for GLU. a Optimized to have an incubation period of 3 h and an overnight incubation using 1% GLU on a 1% APTES-modified surface. b Detection of 125 nM p24 by three different approaches, conventional, APTES-GLU-p24 (method 1), and APTES-GLU-premixed p24 (method 2)Back to article page