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Fig. 1 | Nanoscale Research Letters

Fig. 1

From: Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field

Fig. 1

Overview of magnetofection using PEI-SPIO-NPs/pDNA complex. Lined polyethyleneimine (LPEI) and superparamagnetic iron oxide nanoparticles (SPIO-NPs) are associated by the reaction of dehydration condensation. For this purpose, SPIO-NPs were coated with LPEI, i.e., the LPEI is tightly bound to the surface of SPIO-NPs and they form PEI-SPIO-NPs together. If PEI-SPIO-NPs are mixed with naked pDNA, negatively charged pDNA binds to positively charged PEI-SPIO-NPs via electrostatic adsorption. Cells are incubated with the PEI-SPIO-NPs/pDNA complexes in the presence of a magnetic field which attracts the complexes toward the cells. The result of magnetofection is that essentially all cells come into contact with vectors and a high percentage of cells are rapidly transfected

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