Fig. 6

Intracellular tracking of PEI-SPIO-NPs/pDNA complexes in the presence of uniform magnetic field (uniform MF) or non-uniform magnetic field (non-uniform MF) and PEI-NPs/pDNA complexes without magnetic field (No MF) at 6, 12, and 24 h post-transfection to MG-63 osteoblasts. Confocal images were obtained from three channels and overlaid: red indicates RBITC-PEI-SPIO-NPs/pDNA complexes (excitation: 554 nm; emission: 576 nm), green represents lysosomes stained with Lysotracker-DND26 and the homogeneous green in the cytoplasm implies GFP expression (excitation: 443 nm; emission: 505 nm), blue signifies the nuclei stained by the Hochest 33342. (excitation: 359 nm; emission: 461 nm)