Fig. 4

The effect of PL and released PL from nanofiber mats on the viability of various CCA cells. a HuCC-T1, b SNU1196, c SNU478, and d SNU245 cholangiocarcinoma cells. 2 × 10⁴ cells in 96-well plates were exposed to PL or released PL from nanofibers for 2 days. For treatment of released PL, PL-incorporated nanofiber disks were immersed into PBS and then release experiment was performed for 5 and 15 days with or without 10 mM H₂O₂. The medium was exchanged until 3 days as similar with drug release study. After that, the medium was harvested between 5 and 15 days of release experiment. This solution was used to investigate the comparison of anticancer activity of PL itself and released PL