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Table 1 HPLC-ESI-MS/MS conditions for analysis of Dox

From: Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro

Chromatographic conditions

 Column

Agilent Eclipse XDB-C18

 Column temperature

40 °C

 Mobile phase

Acetonitrile, 0.1% formic acid in H2O (80:20, v:v)

 Flow rate

0.3 ml/min

 Run time

17 min

 Injection volume

3 μl

MS/MS conditions

 Ionization source

ESI

  • Desolvation line temperature

250 °C

  • Heat block temperature

400 °C

 Target molecular ion

544.2 [M]+ m/z

 Product ions

130.2, 361.1 m/z

 Time window

0–17 min

 Dwell time

0.2 s

 Interface voltage

4.5 kV

 Nebulizing gas flow

3 l/min

 Drying gas flow

15 l/min

 LOD

0.005 μM

 LOQ

0.015 μM

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