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Table 1 HPLC-ESI-MS/MS conditions for analysis of Dox

From: Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro

Chromatographic conditions
 Column Agilent Eclipse XDB-C18
 Column temperature 40 °C
 Mobile phase Acetonitrile, 0.1% formic acid in H2O (80:20, v:v)
 Flow rate 0.3 ml/min
 Run time 17 min
 Injection volume 3 μl
MS/MS conditions
 Ionization source ESI
  • Desolvation line temperature 250 °C
  • Heat block temperature 400 °C
 Target molecular ion 544.2 [M]+ m/z
 Product ions 130.2, 361.1 m/z
 Time window 0–17 min
 Dwell time 0.2 s
 Interface voltage 4.5 kV
 Nebulizing gas flow 3 l/min
 Drying gas flow 15 l/min
 LOD 0.005 μM
 LOQ 0.015 μM