Table 1 HPLC-ESI-MS/MS conditions for analysis of Dox
From: Complexation with C60 Fullerene Increases Doxorubicin Efficiency against Leukemic Cells In Vitro
Chromatographic conditions | |
Column | Agilent Eclipse XDB-C18 |
Column temperature | 40 °C |
Mobile phase | Acetonitrile, 0.1% formic acid in H2O (80:20, v:v) |
Flow rate | 0.3 ml/min |
Run time | 17 min |
Injection volume | 3 μl |
MS/MS conditions | |
Ionization source | ESI |
• Desolvation line temperature | 250 °C |
• Heat block temperature | 400 °C |
Target molecular ion | 544.2 [M]+ m/z |
Product ions | 130.2, 361.1 m/z |
Time window | 0–17 min |
Dwell time | 0.2 s |
Interface voltage | 4.5 kV |
Nebulizing gas flow | 3 l/min |
Drying gas flow | 15 l/min |
LOD | 0.005 μM |
LOQ | 0.015 μM |