Fig. 4

Anti-MIF IF images of liver samples from the saline (negative control) and alcohol-induced steatohepatitis groups stained with green fluorescent compounds (ALEXA, AuNPs, or both) and DAPI (blue) for nuclei. a Negative control stained with ALEXA standard protocol (A1) with anti-MIF and ALEXA (green). b Negative control stained with ALEXA modified protocol (A2) with anti-MIF and ALEXA. c Negative control stained with nanoparticle protocol 1 (NP1) with anti-MIF and AuNPs (green) in place of ALEXA. d Negative control stained with nanoparticle protocol 2 (NP2) with anti-MIF and AuNPs in place of ALEXA. e Positive control stained with A1. f Positive control stained with A2. g Positive control stained with NP1. h Positive control stained with NP2. i Positive control stained with nanoparticle protocol 3 (NP3) with anti-MIF + AuNPs with 1:200 ALEXA. j Positive control stained with NP3 with a 1:400 dilution of ALEXA. k Positive control stained with NP3 with a 1:800 dilution of ALEXA. Magnifications, × 200. Scale bar = 50 μm