Fig. 5

COX-2 and MIF antibody-conjugated gold nanoparticles (AuNPs) have high affinity on the surface of M2 macrophages. a–c RAW 264.7 cells were stimulated with IL-4 (20 ng/ml) for 24 h, followed by flow cytometry analysis to quantify the amount of CD163, an M2 macrophage marker, and CD86, an M1 marker. d, f Either COX-2 and MIF antibody-conjugated gold nanoparticles or e, f ALEXA 488 showed higher fluorescence intensity in M2 macrophages when compared to unstained samples. Data are expressed as mean ± SD, ^#p > 0.05, ***p < 0.001, p < 0.0001. Representative flow data shown are from experiments independently performed at least three times