Fig. 6

Fluorescence emission spectra of AuNPs excited at 320 nm in the presence of anti-COX-2 (a), anti-MIF (b), and ALEXA (c). The TEM image of the spherical nanoparticles used in this study, showing its size and distribution (d). Excitation and emission slits were 10 nm. The concentration of AuNPs was held constant at 29.6 ng/ml⁻¹. The concentrations of antibody are 100 ng/ml⁻¹ (blue curve), 150 ng/ml⁻¹ (red curve), and 200 ng/ml⁻¹ (green curve)