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Table 1 Comparison of immunofluorescence protocols based on ALEXA and AuNPs

From: Environmentally compatible bioconjugated gold nanoparticles as efficient contrast agents for inflammation-induced cancer imaging

  Deparaffinization, rehydration and PBS washes. Antigen Retrieval - block solution Primary antibodies incubation ALEXA/ AuNPs incubation PBS washes and slides assembling with DAPI
Immunofluorescence Protocols
 A1 1h 3,8h (230 min) 18h ALEXA (1h) 20 min
 A2 1h 3,8h (230 min) 30 min ALEXA (30 min) 20 min
 NP1 1h 3,8h (230 min) 30 min AuNPs (30 min) 20 min
 NP2 1h 3,8h (230 min) 18h AuNPs (1h) 20 min
 NP3 1h 3,8h (230 min) 18h ALEXA + AuNPs (1h) 20 min