Table 1 Comparison of immunofluorescence protocols based on ALEXA and AuNPs
| Deparaffinization, rehydration and PBS washes. | Antigen Retrieval - block solution | Primary antibodies incubation | ALEXA/ AuNPs incubation | PBS washes and slides assembling with DAPI | |
|---|---|---|---|---|---|
| Immunofluorescence Protocols | |||||
| A1 | 1h | 3,8h (230 min) | 18h | ALEXA (1h) | 20 min |
| A2 | 1h | 3,8h (230 min) | 30 min | ALEXA (30 min) | 20 min |
| NP1 | 1h | 3,8h (230 min) | 30 min | AuNPs (30 min) | 20 min |
| NP2 | 1h | 3,8h (230 min) | 18h | AuNPs (1h) | 20 min |
| NP3 | 1h | 3,8h (230 min) | 18h | ALEXA + AuNPs (1h) | 20 min |