Table 2 Comparison of flow cytometry protocols based on ALEXA and AuNPs
Overral time | 2% Formaldehyde fixation (10 min, 37°C) | Primary antibody incubation | Incubation with fluorochromeconjugated substances | Washes | Resuspension and analysis | |
---|---|---|---|---|---|---|
Flow Cytometry Protocols | ||||||
Standard | 4h | Yes | 1h | ALEXA 488 diluted in PBA - 1 h | 2x | Yes |
AuNPs | 1h | No | 30 min | AuNPs - 30 min | None | Yes |