Fig. 3

NaOH pretreatment is the optimal method for detecting nAg100 in various organs. As in Fig. 2, the heart, kidney, lung and spleen homogenates were mixed with nAg100 and incubated with NaOH solution. After 3 h, a residue rates (black and white bars represent the residue rates in NaOH- or PBS-treatment, respectively), b recovery rates (black and white bars represent the rate of Ag detected as particles and as ions, respectively), c average particle diameters shown in a beeswarm chart, and d particle size distribution shown in a beeswarm chart were analyzed by sp-ICP-MS analysis in each tissue sample. The results are expressed as mean ± SD (n = 3)