Scheme 1
From: Potent anti-tumor immunostimulatory biocompatible nanohydrogel made from DNA
The schematic illustration on the preparation and cellular uptake of CpG-MCA gels and CpG-RCA gel. Long single-stranded DNA with a phosphorylated group at 5' end were mixed with primers composed of CpG motifs were first annealed and ligated by T4 DNA ligase to form a circular template. RCA and MCA reaction was performed by phi29 DNA polymerase to generate a large amount of sequential concatemer DNA and convolve like many nanoflowers. Gels can then be uptake by macrophages and stimulate the secretion of cytokines