Table 2 Precision, matrix effect, extraction recovery and stability of daidzein, and IS detected by LC–MS/MS method (n = 6)
From: Preparation and Pharmacokinetic Study of Daidzein Long-Circulating Liposomes
Components | Concentration (ng/mL) | Within-batch precision (%, RSD) | Between-batch precision (%, RSD) | Matrix effect (%) | Extraction recovery (%) |
Daidzein | 10 | 7.21 | 4.68 | 69.97 | 114.37 |
500 | 6.48 | 6.32 | 87.64 | 93.21 | |
800 | 4.45 | 5.98 | 86.37 | 91.81 | |
IS | 100 | – | – | 84.86 | 96.59 |
Components | Concentration (ng/mL) | Stability (%, RSD) | |||
Freeze and thaw three times | Placed at room temperature for 24 h | Freezing at − 20 °C for 5 days | |||
Daidzein | 10 | 9.87 | 8.73 | 3.78 | |
500 | 3.42 | 6.95 | 6.89 | ||