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Table 2 Precision, matrix effect, extraction recovery and stability of daidzein, and IS detected by LC–MS/MS method (n = 6)

From: Preparation and Pharmacokinetic Study of Daidzein Long-Circulating Liposomes

Components Concentration (ng/mL) Within-batch precision (%, RSD) Between-batch precision (%, RSD) Matrix effect (%) Extraction recovery (%)
Daidzein 10 7.21 4.68 69.97 114.37
500 6.48 6.32 87.64 93.21
800 4.45 5.98 86.37 91.81
IS 100 84.86 96.59
Components Concentration (ng/mL) Stability (%, RSD)
Freeze and thaw three times Placed at room temperature for 24 h Freezing at − 20 °C for 5 days
Daidzein 10 9.87 8.73 3.78
500 3.42 6.95 6.89