Fig. 2

Flow cytometric quantification of the percentage of CD11b⁺Gr-1⁺ cells after treatment with FMT at 250, 500, 1000, 2000 μg/mL in vitro. a MDSC cell viability was detected using CCK8 assays after treatment with various concentrations of FMT for 24 h. b, c Bone marrow cells isolated from C57BL/6 mice were cultured for 4 days with GM-CSF and IL-6 in the presence of different concentrations of FMT, and the cell phenotypes were evaluated by flow cytometry. All data are representative of three independent experiments for each experimental group and are displayed as the means ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by an unpaired Student’s t test versus cells treated with vehicle alone