Fig. 3

FMT alters the function of MDSCs in vitro. Bone marrow–derived MDSCs treated with vehicle or FMT for 24 h. a–h Arg-1, S100A8, S100A9, and p47phox gene expression from treated MDSCs as measured by quantitative RT-PCR. i BM-derived MDSCs were incubated with FMT for 24 h and ROS production was detected by FCM. j Quantitative data of i. k Subpopulations of MDSCs in the FMT-treated and control groups. l FACS analysis of the percentages of G-MDSCs and M-MDSCs. All data are representative of three independent experiments for each experimental group and are displayed as the means ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001 as determined by an unpaired Student’s t test versus cells treated with vehicle alone