Fig. 5

The impact of silencing BRAF gene on Hepa1-6 cells by GAL-GNR-siBRAF. a Proliferation of Hepa1-6 Cells. Hepa1-6 cells were transfected with GAL-GNR-siBRAF, GAL-GNR-siGL2, or PBS, and the cell proliferation was measured by MTT assay (n = 5). The error bar represents the standard deviation of three experiments (*p < 0.05). b Scratch test of Hepa1-6 cells. Hepa1-6 cells were transfected for 24 h as described above and then scraped with a 10 μL tip. The cell scratch images were taken at different time points (scale bar = 200 μm). c and e Migration and invasion of Hepa1-6 cells. Hepa1-6 cells were transfected for 24 h as described above. Cell migration and invasion ability was determined by transwell assay, and imagines were taken at different time points (scale bar = 200 μm). d and f Columnar statistical analysis of cell migration and invasive capacity (***p < 0.001)