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Fig. 4 | Nanoscale Research Letters

Fig. 4

From: RETRACTED ARTICLE: Restored microRNA-133a-3p or Depleted PSAT1 Restrains Endothelial Cell Damage-Induced Intracranial Aneurysm Via Suppressing the GSK3β/β-Catenin Pathway

Fig. 4

Highly expressed miR-133a-3p and lowly expressed PSAT1 inhibit apoptosis and promote proliferation and migration of IA ECs. a Detection of apoptosis of ECs by flow cytometry. b Detection of apoptosis rate of ECs in each group. c Bax and Bcl-2 expression in ECs detected by RT-qPCR. d Protein band of Bax and Bcl-2 expression. e Bax and Bcl-2 protein expression in ECs detected by western blot analysis. f MTT assay was used to detect proliferation activity of ECs in each group. g RT-qPCR was used to detect the expression of Ki-67 and CyclinD1 in each group of ECs. h Protein band of Ki-67 and CyclinD1 expression. i Ki-67 and CyclinD1 protein expression in ECs detected by western blot analysis. j Detection of the migration of ECs in each group by scratch test. k Statistical results of endothelial cell migration in each group. N = 3, *P < 0.05 vs. the control group. #P < 0.05 vs. the mimic NC group. &P < 0.05 vs. the si-NC group. +P < 0.05 vs. the miR-133a-3p mimic group. Measurement data were depicted as mean ± standard deviation; data were assessed by one-way analysis of variance followed by Tukey’s post hoc test

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