Fig. 2
From: Kinetics and Specificity of HEK293T Extracellular Vesicle Uptake using Imaging Flow Cytometry
EV internalization inhibition assays. HEK293T cells were co-cultured with HEK293T EVs under various conditions. Control (37 °C) refers to co-culture in physiological 37 °C environment. Cold refers to co-culture in a 4 °C environment. Fixed inhibition refers to an assay where recipient cells were PFA fixed prior to co-culture. a Representative IFC images of recipient cells. Column 1, BF, signifies bright field. Column 2, GFP, signifies green fluorescent protein (488 nm excitation laser), and Column 3 signifies a merge of BF and GFP. Control shows positive GFP representing EV internalization. b–d Quantification of inhibition assays compared to controls via spot count, mean fluorescence intensity, and maximum pixel. Bars represent mean ± SEM; N = 3; one-way ANOVA followed with Tukey’s post hoc test compared with control. *p < 0.05; ***p < 0.01