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Fig. 3 | Nanoscale Research Letters

Fig. 3

From: F-actin Regulates Osteoblastic Differentiation of Mesenchymal Stem Cells on TiO2 Nanotubes Through MKL1 and YAP/TAZ

Fig. 3

TiO2 nanotubes enhanced osteogenic gene expression of BMSCs. a ALP staining of the smooth titanium substrate and five different nanotube substrates. The cells were induced with osteogenic medium for 7 days. b Statistical analysis of the staining area was performed using ImageJ. c Osteogenesis-associated proteins (RUNX2, and OSX) and F-actin in MSCs were analyzed by Western blotting at day 7. The mRNA expression of RUNX2 (d), ALP (e), OCN (f), and OSX (g) at days 3 and 7, analyzed by qRT-PCR. NT the nanotube group. Data represent the mean ± SD of three samples. *P < 0.05, **P < 0.01, and ***P < 0.001

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