Fig. 2

M1 macrophage-derived exosomes deliver miR-326 to HCC cells and affect miR-326 expression in HCC cells. a Uptake of M1 macrophages derived exosomes by HepG2 cells. b Uptake of M1 macrophages-derived exosomes by SMMC-7721 cells. c Comparison of miR-326 expression of macrophage exosomes in each group before and after INF-γ and LPS induction. d miR-326 expression in HCC cell lines (BEL-7404, HepG2, SMMC-7721, QGY-7703) and human normal hepatocyte HL-7702 cell line detected by RT-qPCR. e RT-qPCR detected the effect of miR-326 mimic on miR-326 expression in HepG2 cells. f RT-qPCR detected the effect of miR-326 inhibitor on miR-326 expression in SMMC-7721 cells. g RT-qPCR detected the effects of miR-326 mimic-transfected M1 macrophage-derived exosomes on miR-326 expression in HepG2 cells. h RT-qPCR detected the effects of miR-326 inhibitor-transfected M1 macrophage-derived exosomes on miR-326 expression in SMMC-7721 cells. In panel c, *P < 0.05 versus the M0 macrophage; In panel d, *P < 0.05 versus HL-7702 cells; In panel e, *P < 0.05 versus the NC-mimic group; In panel f, *P < 0.05 versus the NC-inhibitor group; In panel g, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-mimic group; In panel h, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-inhibitor group. Measurement data were depicted as mean ± standard deviation (N = 3), comparisons among multiple groups were conducted by one-way analysis of variance