Fig. 5

M1 macrophage exosomal miR-326 promotes apoptosis of HCC cells. a, b Flow cytometry detected the effect of transfection of miR-326 mimic on HepG2 cell cycle; c, d flow cytometry detected the effect of transfection of miR-326 mimic on HepG2 cell apoptosis; e, f flow cytometry detected the effect of transfection of miR-326 inhibitor on SMMC-7721 cell cycle; g, h flow cytometry detected the effect of transfection of miR-326 inhibitor on SMMC-7721 cell apoptosis; i, j flow cytometry detected the effects of co-culture of with miR-326 mimic-transfected M1 macrophage-derived exosomes on HepG2 cell cycle; k, l flow cytometry detected the effect of co-culture with miR-326 mimic-transfected M1 macrophage-derived exosomes on HepG2 cell apoptosis; m, n flow cytometry detected the effect of co-culture with miR-326 inhibitor-transfected M1 macrophage-derived exosomes on SMMC-7721 cell cycle; o, p, flow cytometry detected the effect of co-culture with miR-326 inhibitor-transfected M1 macrophage-derived exosomes on SMMC-7721 cell apoptosis. In panel b and d, *P < 0.05 versus the NC-mimic group; In panel f and h, *P < 0.05 versus the NC-inhibitor group; In panel j and l, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-mimic group; In panel n and p, *P < 0.05 versus the control group, #P < 0.05 versus the Exo-NC-inhibitor group . Measurement data were depicted as mean ± standard deviation (N = 3), comparisons among multiple groups were conducted by one-way analysis of variance