Table 1 Primer sequence
Gene | Sequence (5′ → 3′) |
|---|---|
miR-185-3p | F: 5′-GGTGGAGAGAAAGGCAGT-3’ |
R: 5′-TGCGTGTCGTGGAGTC-3’ | |
U6 | F: 5′-CTCGCTTCGGCAGCACA-3’ |
R: 5′-AACGCTTCACGAATTTGCGT-3’ | |
SOX2 | F: 5′-CGCCCCCAGCAGACTTCACA-3’ |
R: 5′-CTCCTCTTTTGCACCCCTCCCATTT-3’ | |
CCAT1 | F: 5′-CATTGGGAAAGGTGCCGAGA-3’ |
R: 5′-ACGCTTAGCCATACAGAGCC-3’ | |
FOXP3 | F: 5′-GTGGCCCGGATGTGAGAAG-3’ |
R: 5′-GGAGCCCTTGTCGGATGATG-3’ | |
GAPDH | F: 5′-CTGGGCTACACTGAGCACC-3’ |
R: 5′-AAGTGGTCGTTGAGGGCAATG-3’ |