Fig. 2
Expression of GPC3 and uptake of hGC33-coumarin6-NP in Li-7 and HepG2 cells. The cells, inoculated in the culture plate, were washed with PBS and incubated with 100 μg/ml hGC33-coumarin6-NP in DMEM for 2, 4, and 8 h. Nuclei were stained with DAPI, and the cells were fixed and detected by confocal laser scanning microscopy. GPC3 was not detected in Li-7 cells, but was highly expressed in HepG2 cells as detected by immunocytochemistry. The scale bar indicates 50 μM