Fig. 3

Inhibited miR-301 or overexpressed PTEN restricts viability of ESCC cells; elevated miR-301 or reduced PTEN promotes viability of ESCC cells. a Number of colonies in KYSE-30 cells after transfection detected using colony formation assay after miR-310 downregulation or PTEN upregulation; b colony formation ability of KYSE-30 cells after transfection detected using colony formation assay after miR-310 downregulation or PTEN upregulation; c viability of KYSE-30 cells after transfection detected using CCK-8 assay after miR-310 downregulation or PTEN upregulation; d number of colonies in Eca109 cells after transfection detected using colony formation assay after miR-310 upregulation or PTEN downregulation; e colony formation ability of Eca109 cells after transfection detected using colony formation assay after miR-310 upregulation or PTEN downregulation; f viability of Eca109 cells after transfection detected using CCK-8 assay after miR-310 upregulation or PTEN downregulation; *P < 0.05 versus the inhibitor-NC group; & P < 0.05 versus the oe-NC group; ^#P < 0.05 versus the miR-301 inhibitor + si-NC group; a P < 0.05 versus the mimic-NC group; b P < 0.05 versus the si-NC group; c P < 0.05 versus the miR-301 mimic + oe-NC group, N = 3. Data were expressed as mean ± standard deviation, and ANOVA was used for comparisons among multiple groups