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Fig. 6 | Nanoscale Research Letters

Fig. 6

From: Quantitative evaluation of cellular internalization of polymeric nanoparticles within laryngeal cancer cells and immune cells for enhanced drug delivery

Fig. 6

Microscopic examination of cellular internalization of 1 µm PLGA particles in monoculture and co-culture cells. Single type of cells or mixed cells was treated with 1 µm PLGA particles for 24 h at a concentration of 10 µg/mL. Cells were observed under bright-field (a, d, g) and fluorescent channels (b, e, h) with particles observed at the green channel and cell nuclei in the blue channel after stained with Hoechst. A large amount of particle uptake and tremendous accumulation of 1 µm PLGA particles can be visualized in magnified images (c, f, i) for monoculture THP-1 (yellow arrows) and UM-SCC-17A laryngeal cancer cells (red arrows). In the co-culture system, 1 µm PLGA particles were still efficiently uptake by macrophages, while cancer cells had insufficient particle ingestion

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