TY - JOUR AU - Lee, Sang Hoon AU - Kim, Hyun-Ouk AU - Kang, Jung-Taek PY - 2021 DA - 2021/03/31 TI - Optimization of Nano-encapsulation on Neonatal Porcine Islet-like Cell Clusters Using Polymersomes JO - Nanoscale Research Letters SP - 53 VL - 16 IS - 1 AB - Researches proving methods for nano-encapsulation of neonatal porcine islet-like cell clusters (NPCCs) using polymersomes (PSomes) formed using polymers of polyethylene glycol-block-poly lactide. Herein, our studies present efficient nano-encapsulation procedure with minimal damage and loss of NPCCs.We used N-hydroxysuccinimide (NHS) on the N-terminal of PSomes to induce binding of amine groups in the extracellular matrix surrounding NPCCs. F-10 culture medium with bovine serum albumin was used in the nano-encapsulation procedure to minimize damage and loss of NPCCs. Finally, we induced cross-linking between bifunctional PSomes (NHS-/NH2-PSomes). F-10 culture medium containing 0.25% BSA with pH of 7.3 minimized the damage and loss of NPCCs after nano-encapsulation as compared with using basic HBSS buffer (pH 8.0). Also, we induced the efficient nano-encapsulation through conjugation of PSomes using bifunctional PSomes (NHS-/NH2-PSomes). SN - 1556-276X UR - https://doi.org/10.1186/s11671-021-03512-3 DO - 10.1186/s11671-021-03512-3 ID - Lee2021 ER -