Fig. 3
From: Optimization of Nano-encapsulation on Neonatal Porcine Islet-like Cell Clusters Using Polymersomes
Comparisons of coating efficiency after 0.25% BSA addition in nano-encapsulation reaction buffers for increase the recovery rate. Comparisons of coating efficiency after 0.25% BSA addition in nano-encapsulation reaction buffers for increase the recovery rate. a Coating efficiency and selective permeability of nano-encapsulated NPCCs with NHS-PSome in F-10 or F-10 with 0.25% BSA (BF; bright field, Mid; middle image using CLSM of DiD-conjugated NHS-PSome nano-encapsulated NPCCs, FITC-conjugated dextran; middle image using CLSM of FITC-conjugated dextran in NHS-PSome nano-encapsulated NPCCs). Blue in Mid represent the cell through DAPI staining. Scale bars are 200 (BF and DiD) and 100 (Mid and FITC-conjugated dextran) μm; b MFI of nano-encapsulated NPCCs using DiD-conjugated NHS-PSome; c Recovery rate of NPCCs after nano-encapsulation in F-10 (n = 12) or F-10 with 0.25% BSA (n = 12). Data represent the mean ± S.D. *p < 0.05 versus F-10