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Fig. 7 | Nanoscale Research Letters

Fig. 7

From: Potential Osteoinductive Effects of Hydroxyapatite Nanoparticles on Mesenchymal Stem Cells by Endothelial Cell Interaction

Fig. 7

HIF-1α promoted osteogenic differentiation of hWJ-MSCs via the ERK1/2 pathway. hWJ-MSCs were incubated in osteogenic medium with or without PD98059 for 14 d. a Extracellular calcium deposition was visualized via ARS staining. b ALP activity of hWJ-MSCs was assessed via ALP staining. Scale bars: 200 μm. c Quantitative analysis of extracellular calcium matrix. *P < 0.05; **P < 0.01; ***P < 0.001 versus control; #P < 0.05; ###P < 0.001 versus the np20 group. Cells incubated in osteogenic medium without HAPs and PD98059 treatment were used as the control group. Abbreviations: HAPs hydroxyapatite particles, m-HAP micro-sized HAP particles, hWJ-MSCs human umbilical cord Wharton’s jelly-derived mesenchymal stem cells, HUVECs human umbilical vein endothelial cells, ERK extracellular signal-regulated kinase, HIF-1α hypoxia-inducible factor 1α, ALP alkaline phosphatase

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