Fig. 8

Two-stage cell-lineage models. hWJ-MSCs were incubated in a defined concentration of HIF-1α for the indicated times. a Relative differentiation rates of hWJ-MSCs at different concentrations of HIF-1α, b relative ALP activity (differentiation degrees of hWJ-MSCs) and relative osteoblast cells on different culture days. c Three-dimensional surface of differentiation degree evolving with time and HIF-1α. Cell number with an initial seeding density of 1500 cells per well (in a 96-well plate), as well as d 0, e 375, and f 1500 pg/mL HIF-1α. Abbreviations: hWJ-MSCs human umbilical cord Wharton’s jelly-derived mesenchymal stem cells, HIF-1α hypoxia-inducible factor 1α, ALP alkaline phosphatase