Table 1 Primers used for real-time RT-PCR
Target gene | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|
RUNX-2 | TGGTTACTGTCATGGCGGGTA | TCTCAGATCGTTGAACCTTGCTA |
Col I | TGGTGGAGCAGCAAGAGCAA | CAGTGGACAGTAGACGGAGGAAA |
OCN | CACTCCTCGCCCTATTGGC | CCCTCCTGCTTGGACACAAAG |
GAPDH | CTGGTAAAGTGGATATTGTTGCCAT | TGGAATCATATTGGAACATGTAAACC |
ALP | GGACATGCAGTACGAGCTGA | GTCAATTCTGCCTCCTTCCA |
NANOG | CAGAAGGCCTCAGCACCTAC | ATTGTTCCAGGTCTGGTTGC |
OCT3/4 | GTGGAGAGCAACTCCGATG | TGCAGAGCTTTGATGTCCTG |
SOX2 | CCAAGACGCTCATGAAGAAG | TGGTCATGGAGTTGTACTGC |
GAPDH | CTGGTAAAGTGGATATTGTTGCCAT | TGGAATCATATTGGAACATGTAAACC |