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Fig. 4 | Nanoscale Research Letters

Fig. 4

From: Synthesis of SPIO Nanoparticles and the Subsequent Applications in Stem Cell Labeling for Parkinson’s Disease

Fig. 4

The synthesized USPIO-PAA/PEG NPs show good biocompatibility and labeling capability. a, b HADSCs or iPSCs were incubated with USPIO-PAA/PEG NPs at varying concentrations (equivalent to Fe concentrations at 0, 10, 20, 40, 80 and 160 μg Fe/mL) for 24, 48, and 72 h, and the cell viability was determined by MTT method and shown in a, b, respectively. There is no significant difference among the viabilities at different NPs concentration (n = 5) or incubation time (n = 5). c Flow cytometry analysis showed that the EdU incorporation rates were similar in the HADSCs incubated in the presence or absence of USPIO-PAA/PEG (160 μg Fe/mL for 72 h). HADSCs did not treated with EdU were served as negative control. d USPIO-PAA/PEG at 10 μg Fe/mL and incubation time for 2 h resulted in almost 100% labeling efficiency of HADSCs, as evaluated by Prussian blue staining. Note that USPIO-PAA/PEG at 0.1 μg Fe/mL (2 h incubation time) produced light but uniform PB stain in the HADSCs. Numerical data are presented as mean ± SD. The bar represents 20 μm

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