Fig. 6

HADSCs characterization. a A representative image under the phrase contrast microscope showing that HADSCs has a typical spindle-like phenotype. b–g HADSCs were incubated with antibodies conjugating with different dyes against CD45, CD105, CD34 and CD90 markers and subjected to flow cytometric analysis. The scatter diagrams are shown in b, c, and the histograms are shown in d–g. Note that most of the cells were expressed CD90 and CD105, but not CD34 and CD45. h Adipogenic differentiation and osteogenic differentiation of HADSCs versus HEK293 cells are evaluated by Oil Red O or Alizarin Red Staining, respectively. The bar represents 20 μm