Fig. 7
From: Brain-Targeted Polysorbate 80-Emulsified Donepezil Drug-Loaded Nanoparticles for Neuroprotection
a Raw MST data. The fluorescently labeled molecules were observed for 5 s. At this time, the infrared laser was turned on, and a small part of the capillary tube was heated to 2–5 °C. The molecules migrated along a thermal gradient, resulting in changes in fluorescence intensity. When the infrared laser is turned off, the molecules diffuse along the concentration gradient. b The binding curve is generated by the difference between the initial fluorescence intensity and the intensity in the presence of heat, and the curve conforms to the standard 1:1 binding model