Fig. 3

Calculated cell area, cell circularity and cell aspect ratio for U2OS cells expressing fluorescent LifeActGFP and TagRFP-vinculin fusion proteins on different surfaces imaged 24 h and 48 h post-transfection. The image detection was performed based on the intensity of the LifeActGFP signal. Each grey point correspond to one cell and the box plots shown median values (Q2) as well as the first (Q1) and third quartile (Q3). Statistical differences between the distributions were assessed using the Mann–Whitney non-parametric test