Fig. 5

Example cell on 2000 nm pillar array expressing A LifeActGFP (green) and B TagRFP-vinculin (red). Superimposed on both figures is the detected cell edge determined by using the signal from expression of LifeActGFP (as shown in A). C Shows a distance map from the detected cell edge as well as detected FAs. The shortest distance (denoted with d in figure) from each segmented vinculin spot (white areas) to the cell periphery (indicated with a solid white line) was calculated for all FAs in the images