Fig. 3From: Gold Nanobiosensor Based on the Localized Surface Plasmon Resonance is Able to Diagnose Human Brucellosis, Introducing a Rapid and Affordable MethodOptimizing coating of thioglycolic acid on gold nanoparticles. As the figure reveals, there is no significant alteration in absorbance after 24 h of incubation despite the elongation of adjacent time. Therefore, 24 h was selected as the optimized time for coating of thioglycolic acid on GNPs. The Y-axis shows the absorbance intensity at 530 nm (max absorbance of nanoparticles). The experiments were done in triplicates. Data are presented as mean ± SEM. a.u.: Absorbance unitBack to article page