Skip to main content
Fig. 2 | Nanoscale Research Letters

Fig. 2

From: Black Phosphorus Nanoparticles Promote Osteogenic Differentiation of EMSCs Through Upregulated TG2 Expression

Fig. 2

Identification of the ectodermal mesenchymal stem cells (EMSCs). A The nestin positive EMSCs (cy3, red) were located in the lamina propria under the respiratory epithelium of the nasal septum; B A phase-contrast image showed that the cultured EMSCs at the third passage appeared as fibroblastic-like cells and proliferated rapidly on plastic plates; C The osteogenic differentiated EMSCs in osteogenic medium were stained by alizarin red; D The adipogenic differentiated EMSCs in adipogenic-induction medium were stained by with oil red-O; E, F Immunofluorescence staining of the neural crest cell markers showed that almost all of the EMSCs expressed nestin and vimentin. The IgG-Cy3 (red) was used as the second antibody for the immunofluorescence staining and the nuclei were counter-stained with Hoechst 33342 (blue)

Back to article page