Fig. 3

Global m⁶A changes induced by BSA-templated NPs. a Scatter plots showing the variation of m⁶A coverage of individual m⁶A sites in cells treated with BSA-templated NPs. Pair-wise comparison was shown between BSA-Au NPs (left), BSA-CuS NPs (middle), BSA-Gd₂O₃ NPs (right), and controls, respectively. The dashed lines indicated cut-off of log₂FC(BSA-templated NPs/Control) (1 or −1). The numbers of genes with downregulated (log₂FC < -1) or upregulated (log₂FC > 1) m⁶A level was shown. FC, fold change. b Cumulative frequency of m⁶A targeted genes upon BSA-templated NPs exposure. P values were calculated using two-sided Wilcoxon and Mann–Whitney test. c Metagene profile of enrichment of m⁶A-targeted sites across mRNA transcriptome. 5’UTR, 5’ untranslated region; CDS, coding sequence; 3’UTR, 3’ untranslated region. d The distribution (left) and enrichment (right) of m⁶A peaks within different gene regions. Enrichment was determined by the proportion of m⁶A peaks normalized by the length of the region. e Top consensus sequences of m⁶A-targeted motif