Fig. 4

miR-140-5p restoration negates the impacts of up-regulated H19 on OGD-induced BMECs. A The binding site of H19 and miR-140-5p detected by bioinformatics website; B The binding relationship verified by dual luciferase report gene assay; C The binding relationship between H19 and miR-140-5p detected by RNA pull-down assay; D The enrichment level of H19 and miR-140-5p detected by RIP assay; E, F miR-140-5p expression in oe-H19- and miR-140-5p-mimic-transfected and OGD-induced BMECs detected by RT-qPCR; G Viability of oe-H19- and miR-140-5p-mimic-transfected and OGD-induced BMECs detected by MTT assay; H Cell apoptosis of oe-H19- and miR-140-5p-mimic-transfected and OGD-induced BMECs detected by flow cytometry; I Cell migration of oe-H19- and miR-140-5p-mimic-transfected and OGD-induced BMECs detected by Transwell assay; J Angiogenesis of oe-H19- and miR-140-5p-mimic-transfected and OGD-induced BMECs detected by Matrigel method; N = 3. The data were expressed as mean ± standard deviation