Table 2 Liposome particle (LP) based anti-GBM drug delivery system
Drug | Composite | Modification | Detail | Result |
|---|---|---|---|---|
Doxorubicin (DOX) [79] | DOX@CB5005@LP | Peptide CB5005 was employed as the cell-penetrating peptide and NF-κB inhibitor | U87 tumor spheroids were prepared to evaluate penetrating ability Cell livability was determined by MTT assay Xenograft-bearing nude mice (subcutaneous and intracranial models) were utilized to evaluate the anti-tumor efficacy | Augmented cellular uptake, increased tumor spheroid penetration and cytotoxicity Enhanced BBB transport and tumor accumulating Prolonged OS |
Paclitaxel (PTX) [80] | Rg3@PTX@LP | Rg3 was a substitute of cholesterol as the building block for drug delivery liposomes. RG3 assisted membrane penetration and possessed synergistic effect with loaded anti-cancer drugs | The cellular uptake and cytotoxicity of liposomes was assessed in C6 cells Tumor spheroids and Transwell model were prepared in vitro to evaluate the penetration ability of liposome system The TAM-modulatory effect of PTX was assessed Intracranial xenograft models were utilized to evaluate anti-tumor efficacy | Improved glioma targeting by Rg3-glucose transporter interaction Enhanced BBB transport and PTX cellular uptake The reverse of immuno-suppressive glioma microenvironment |
Rapamycin(RPM) [81] | RPM@MTI-31@DVAP@LP | DVAP was applied as tumor homing peptide | The cellular uptake and cytotoxic activity of liposome MTI-31 were evaluated in U87 cells in vitro Xenograft-bearing nude mice (intracranial models) were utilized to evaluate the anti-tumor efficacy | Increased cellular uptake and anti-proliferative effect against tumor cells High selectivity and glioma targeting property Improved median survival time |
Docetaxel (DTX) [82] | DTX@ RI7217@Muscone@LP | RI7217 had high affinity and selectivity for TfR Muscone was a musk ingredient that inhibited the expression of P-gp and MMP-9 | Cell livability was determined by MTT assay U87 tumor spheroids and Transwell model were prepared to evaluate penetrating ability U87-MG glioma implantation in nude mice was employed for imaging and survival monitoring | Enhanced BBB transport and tumor penetration Improved targeting ability and anti-glioma effect in vitro and in vivo |
Doxorubicin [83] | DOX@P1NS@TNC @ SPIONs@LP | P1NS was GBM-specific cell-penetrating peptide. The anti-GBM antibody TNC and superparamagnetic iron oxide provided targeted delivery property | MTS assays were used to assess cytotoxicity BBB model was established to study liposome permeability The U87 tumor-specific cellular uptake was evaluated by CLMS In vitro stability of liposome and hemolysis assay were conducted | Enhanced BBB penetration Specific tumor tissue targeting Controlled release of encapsulated drugs Increased anti-tumor efficacy and high bio-tolerance |
Paclitaxel [84] | PTX@Ang-2@A15@ surviving siRNA@LP | Angiopep-2 could specifically target LPR on BBB surface. A15 was a RNA aptamer that binds to CD133 | CCK8 assay and Annexin V-FITC apoptosis assay were used to evaluate the anti-proliferative activity Xenograft-bearing nude mice (subcutaneous and intracranial models) were utilized to evaluate the anti-tumor efficacy | Induced apoptosis of tumor marginal and internal cells Enhanced BBB transport Synergetic chemo-gene therapeutic effect Inhibition of survivin enhanced PTX activity |