Fig. 2
a Zeta potential of ZIF-8, ZIF-8/ICG, MnO2/ZIF-8/ICG, and FA-EM@MnO2/ZIF-8/ICG. b Size distribution of ZIF-8, ZIF-8/ICG, MnO2/ZIF-8/ICG, and FA-EM@MnO2/ZIF-8/ICG. c Fluorescence spectra of ZIF-8, the ICG solution before loading, and the residual ICG in the supernatant after loading. d In vitro profiles of ICG release from FA-EM@MnO2/ZIF-8/ICG nano-platform under conditions of pH 7.4, pH 5.5, H2O2, GSH and simulated TME. e Temperature changes of PBS (curve 1), 200 μg/mL ZIF-8/ICG (curve 2), 200 μg/mL FA-EM@MnO2/ZIF-8/ICG (curve 3), and 150 μg/mL FA-EM@MnO2/ZIF-8/ICG (curve 4) irradiated with 808 nm irradiation (2.0 W/cm2) for 10 min, and 200 μg/mL FA-EM@MnO2/ZIF-8/ICG (curve 5) irradiated with 808 nm irradiation (1.5 W/cm2) for 10 min. f Temperature changes of 200 μg/mL FA-EM@MnO2/ZIF-8/ICG disperse solution irradiation with 808 nm irradiation (2.0 W/cm2) for five turn on/off cycles. g Photothermal response of FA-EM@MnO2/ZIF-8/ICG solution and H2O treated with an NIR irradiation (808 nm, 1.5 W/cm2) for 600 s and then the irradiation was turned off. Viability of GES-1 cells (h) and 4T1cells (i) upon treated with PBS (1), PBS + NIR (2), ZIF-8/ICG + NIR (3), MnO2/ZIF-8/ICG + NIR (4), and FA-EM@MnO2/ZIF-8/ICG + NIR (5)