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Fig. 3 | Nanoscale Research Letters

Fig. 3

From: An Enzyme-Free Sandwich Amperometry-Type Immunosensor Based on Au/Pt Nanoparticle-Functionalized Graphene for the Rapid Detection of Avian Influenza Virus H9 Subtype

Fig. 3

A Nyquist plots and B the Ret (error bar = RSD, n = 5) obtained from the EIS characterization of electrodes at different modification steps in electrolyte (pH 7.0) containing 5 mM [Fe(CN)6]3−/4−: (a) GCE, (b) GS-Chi-GCE, (c) AIV H9/MAbs-GS-Chi-GCE, (d) BSA-AIV H9/MAbs-GS-Chi-GCE, (e) AIV H9-BSA-AIV H9/MAbs-GS-Chi-GCE. C and D CV of (a) GCE, (b) GS-Chi-GCE, (c) AIV H9/MAbs-GS-Chi-GCE, (d) BSA-AIV H9/MAbs-GS-Chi-GCE and (e) AIV H9-BSA-AIV H9/MAbs-GS-Chi-GCE. The scan rate was 50 mV s−1. E and F Amperometric i–t curves and measured responses of the immunosensor for the detection of 106.37 EID50/mL AIV H9 (a) without labels; (b) with GS-Chi-Au/Pt-AIV H9/PAbs, (c) GS-Chi-AIV H9/PAbs, (d) GS-Chi-Au-AIV H9/PAbs, (e) Chi-Au/Pt-AIV H9/PAbs and (f) GS-Chi-Au/Pt-AIV H9/PAbs as labels; and (g) without incubation with AIV H9 but with GS-Chi-Au/Pt-AIV H9/PAbs as labels at − 0.4 V towards the reduction of 10 mM H2O2 in electrolyte at pH 7.0

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