An Enzyme-Free Sandwich Amperometry-Type Immunosensor Based on Au/Pt Nanoparticle-Functionalized Graphene for the Rapid Detection of Avian Influenza Virus H9 Subtype

Table 2 Comparison of the proposed immunosensor with other methods for AIV H9 detection

Method	Detection time	Detection limit	References
Virus isolation	4 days	1 EID₅₀ mL⁻¹	[6]
RT–PCR	6 h	100 EID₅₀ mL⁻¹	[7]
Real-time RT–PCR	4 h	1 EID₅₀ mL⁻¹	[8]
RT-LAMP	3 h	10 copies per reaction	[9]
ELISA	2 h	10^−2.3 TCID₅₀	[10]
Proposed immunosensor	1.5 h	10^0.82 EID₅₀ mL⁻¹	This study