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Table 3 Results from clinical samples (a); results from the analysis of positive samples (b); and results for the recovery of different concentrations of AIV H9 from clinical samples (c)

From: An Enzyme-Free Sandwich Amperometry-Type Immunosensor Based on Au/Pt Nanoparticle-Functionalized Graphene for the Rapid Detection of Avian Influenza Virus H9 Subtype

(a)

Method

Total number of samples

Number of positive samples

Positivity rate (%)

Proposed immunosensor

98

3

3.06

Virus isolation

98

3

3.06

(b)

No

Results from the proposed immunosensor

Results of virus isolation

Measured concentration (EID50 mL−1)

Average (EID50 mL−1)

RSD (%) (n = 5)

1

60.24, 57.31, 62.17, 63.54, 64.49

61.55

4.63

Positive

2

141.25, 137.86, 143.74, 136.47, 142.18

140.30

2.17

Positive

3

192.67, 188.34,193.28, 187.59, 195.73

191.52

1.80

Positive

(c)

No

Initial AIV H9 concentration in the sample (EID50 mL−1)

Added amount of AIV H9 (EID50 mL−1)

Total found

Recovery rate (%) (n = 5)

Average (EID50 mL−1)

RSD (%) (n = 5)

1

61.55

100.00

169.03

3.47

104.63

2

61.55

150.00

206.93

2.73

97.82

3

140.30

200.00

326.74

4.07

96.02

4

140.30

250.00

381.37

2.17

97.71

5

191.52

300.00

497.31

4.18

101.18

6

191.52

400.00

584.62

3.75

98.83

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