Skip to main content


We're creating a new version of this page. See preview

  • Nano Express
  • Open Access

Fabrication of DNA micropatterns on the polycarbonate surface of compact discs

Nanoscale Research Letters20062:69

  • Received: 24 October 2006
  • Accepted: 8 November 2006
  • Published:


DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, contact angle titrations were carried out on the PC surfaces. The surface morphologies of PC surface were measured by Tapping-mode Atomic Force Microscopy (AFM). As the surface as “anchor” to attach the amine-modified ssDNA by covalent conjugates via amide bonds, the results of fluorescence spectroscopy showed that the fluorescein labeled complementary ssDNA can be used for detection the ssDNA immobilized on the patterned CD polycarbonate substrate.


  • DNA
  • Compact disc
  • UV/ozone
  • Hybridization


Authors’ Affiliations

Center for Advanced Materials & Biotechnology, Research Institute of Tsinghua University in Shenzhen, Shenzhen, 518057, P.R. China


  1. Pirrung M: Angew. Chem., Int. Ed. 2002, 41: 1276. COI number [1:CAS:528:DC%2BD38Xjt1ertbk%3D] 10.1002/1521-3773(20020415)41:8<1276::AID-ANIE1276>3.0.CO;2-2View ArticleGoogle Scholar
  2. Cheung VG, Morley M, Aguilar F, Massimi A, Kucherlapat R, Childs G: Nat. Genet.. 1999, 21: 15. COI number [1:CAS:528:DyaK1MXltlWhsw%3D%3D] 10.1038/4439View ArticleGoogle Scholar
  3. Dubitsky A, Brown J, Brandwein H: BioTechniques. 1992, 13: 392. COI number [1:CAS:528:DyaK3sXhs1er]Google Scholar
  4. H.Z. Yu, Chem. Commun. 2004, 2633 and references thereinGoogle Scholar
  5. Madou MJ, Lee LJ, Daunert S, Lai S, Shih CH: Biomed. Microdevices. 2001, 3: 245. COI number [1:CAS:528:DC%2BD3MXotVyrtLs%3D] 10.1023/A:1011419515576View ArticleGoogle Scholar
  6. Lai S, Wang S, Luo J, Lee J, Yang ST, Madou MJ: Anal. Chem.. 2004, 76: 1832. COI number [1:CAS:528:DC%2BD2cXhsVKks7k%3D] 10.1021/ac0348322View ArticleGoogle Scholar
  7. Kido H, Maquieira A, Hammock BD: Anal. Chim. Acta.. 2000, 411: 1. COI number [1:CAS:528:DC%2BD3cXit1Wjsr0%3D] 10.1016/S0003-2670(00)00776-5View ArticleGoogle Scholar
  8. Alexander I, Houbin Y, Collet J, Hamels S, Demarteau J, Gala JL, Remacle J: BioTechniques. 2002, 33: 435.Google Scholar
  9. La Clair JJ, Burkart MD: Org. Biomol. Chem.. 2003, 1: 3244. COI number [1:CAS:528:DC%2BD3sXntV2ntbo%3D] 10.1039/b306391gView ArticleGoogle Scholar
  10. (a) Y.J. Liu, D. Ganser, A. Schneider, R. Liu, P. Grodzinski. N. Kroutchinina, Anal. Chem. 73, 4196 (2001) (b) Y.J. Liu, C.B. Rauch, Anal. Biochem. 317, 76 (2003)View ArticleGoogle Scholar
  11. A. Welle, E. Gottwald, Biomed Microdevices 4, 33 (2002)View ArticleGoogle Scholar
  12. (a) R.L. McCarley, B. Vaidya, S.Y. Wei, A.F. Smith, A.B. Patel, J. Feng, M.C. Murphy, S.A. Soper, J. Am. Chem. Soc. 127, 842 (2005) (b) Y.C. Xu, B. Vaidye, A.B. Patel, S.M. Ford, R.L. McCarley, S.A. Soper, Anal. Chem. 75, 2975 (2003)View ArticleGoogle Scholar
  13. (a) H.Z. Yu, Anal. Chem. 73, 4743 (2001) (b) H.Z. Yu, A.W. Rowe, D.M. Waugh, Anal. Chem. 74, 5742 (2002)View ArticleGoogle Scholar
  14. (a) J.M. Helt, C.M. Drain, J.D. Batteas, J. Am. Chem. Soc. 126, 628 (2004) (b) P. Hazarika, D. Chowdhury, A. Chattopadhyay, Lab Chip 3, 128 (2003)View ArticleGoogle Scholar
  15. 15. L. Angnes, E.M. Richter, M.A. Augelli, G.H. Kume, Anal. Chem. 72, 5503 (2000)View ArticleGoogle Scholar
  16. 16. J.T.C. Wojtyk, M. Tomietto, R. Boukherroub, D.D.M. Wayner, J. Am. Chem. Soc. 123, 1535 (2001)View ArticleGoogle Scholar
  17. Holmes-Farley SR, Reamey RH, McCarthy TJ, Deutch J, Whitesides GM: Langmuir. 1985, 1: 725. COI number [1:CAS:528:DyaL2MXlvVCiu74%3D] 10.1021/la00066a016View ArticleGoogle Scholar
  18. Joos B, Kuster H, Cone R: Anal. Biochem.. 1997, 247: 96. COI number [1:CAS:528:DyaK2sXisFylt78%3D] 10.1006/abio.1997.2017View ArticleGoogle Scholar


© to the authors 2006