Skip to main content

Fabrication of DNA micropatterns on the polycarbonate surface of compact discs


DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, contact angle titrations were carried out on the PC surfaces. The surface morphologies of PC surface were measured by Tapping-mode Atomic Force Microscopy (AFM). As the surface as “anchor” to attach the amine-modified ssDNA by covalent conjugates via amide bonds, the results of fluorescence spectroscopy showed that the fluorescein labeled complementary ssDNA can be used for detection the ssDNA immobilized on the patterned CD polycarbonate substrate.



  1. Pirrung M: Angew. Chem., Int. Ed. 2002, 41: 1276. COI number [1:CAS:528:DC%2BD38Xjt1ertbk%3D] 10.1002/1521-3773(20020415)41:8<1276::AID-ANIE1276>3.0.CO;2-2

    Article  Google Scholar 

  2. Cheung VG, Morley M, Aguilar F, Massimi A, Kucherlapat R, Childs G: Nat. Genet.. 1999, 21: 15. COI number [1:CAS:528:DyaK1MXltlWhsw%3D%3D] 10.1038/4439

    Article  Google Scholar 

  3. Dubitsky A, Brown J, Brandwein H: BioTechniques. 1992, 13: 392. COI number [1:CAS:528:DyaK3sXhs1er]

    Google Scholar 

  4. H.Z. Yu, Chem. Commun. 2004, 2633 and references therein

  5. Madou MJ, Lee LJ, Daunert S, Lai S, Shih CH: Biomed. Microdevices. 2001, 3: 245. COI number [1:CAS:528:DC%2BD3MXotVyrtLs%3D] 10.1023/A:1011419515576

    Article  Google Scholar 

  6. Lai S, Wang S, Luo J, Lee J, Yang ST, Madou MJ: Anal. Chem.. 2004, 76: 1832. COI number [1:CAS:528:DC%2BD2cXhsVKks7k%3D] 10.1021/ac0348322

    Article  Google Scholar 

  7. Kido H, Maquieira A, Hammock BD: Anal. Chim. Acta.. 2000, 411: 1. COI number [1:CAS:528:DC%2BD3cXit1Wjsr0%3D] 10.1016/S0003-2670(00)00776-5

    Article  Google Scholar 

  8. Alexander I, Houbin Y, Collet J, Hamels S, Demarteau J, Gala JL, Remacle J: BioTechniques. 2002, 33: 435.

    Google Scholar 

  9. La Clair JJ, Burkart MD: Org. Biomol. Chem.. 2003, 1: 3244. COI number [1:CAS:528:DC%2BD3sXntV2ntbo%3D] 10.1039/b306391g

    Article  Google Scholar 

  10. (a) Y.J. Liu, D. Ganser, A. Schneider, R. Liu, P. Grodzinski. N. Kroutchinina, Anal. Chem. 73, 4196 (2001) (b) Y.J. Liu, C.B. Rauch, Anal. Biochem. 317, 76 (2003)

    Article  Google Scholar 

  11. A. Welle, E. Gottwald, Biomed Microdevices 4, 33 (2002)

    Article  Google Scholar 

  12. (a) R.L. McCarley, B. Vaidya, S.Y. Wei, A.F. Smith, A.B. Patel, J. Feng, M.C. Murphy, S.A. Soper, J. Am. Chem. Soc. 127, 842 (2005) (b) Y.C. Xu, B. Vaidye, A.B. Patel, S.M. Ford, R.L. McCarley, S.A. Soper, Anal. Chem. 75, 2975 (2003)

    Article  Google Scholar 

  13. (a) H.Z. Yu, Anal. Chem. 73, 4743 (2001) (b) H.Z. Yu, A.W. Rowe, D.M. Waugh, Anal. Chem. 74, 5742 (2002)

    Article  Google Scholar 

  14. (a) J.M. Helt, C.M. Drain, J.D. Batteas, J. Am. Chem. Soc. 126, 628 (2004) (b) P. Hazarika, D. Chowdhury, A. Chattopadhyay, Lab Chip 3, 128 (2003)

    Article  Google Scholar 

  15. 15. L. Angnes, E.M. Richter, M.A. Augelli, G.H. Kume, Anal. Chem. 72, 5503 (2000)

    Article  Google Scholar 

  16. 16. J.T.C. Wojtyk, M. Tomietto, R. Boukherroub, D.D.M. Wayner, J. Am. Chem. Soc. 123, 1535 (2001)

    Article  Google Scholar 

  17. Holmes-Farley SR, Reamey RH, McCarthy TJ, Deutch J, Whitesides GM: Langmuir. 1985, 1: 725. COI number [1:CAS:528:DyaL2MXlvVCiu74%3D] 10.1021/la00066a016

    Article  Google Scholar 

  18. Joos B, Kuster H, Cone R: Anal. Biochem.. 1997, 247: 96. COI number [1:CAS:528:DyaK2sXisFylt78%3D] 10.1006/abio.1997.2017

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Zhen Wang.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 2.0 International License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Wang, Z., Li, RX. Fabrication of DNA micropatterns on the polycarbonate surface of compact discs. Nanoscale Res Lett 2, 69 (2007).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI:


  • DNA
  • Compact disc
  • UV/ozone
  • Hybridization