Figure 13

Cell type internalising Cy5.5-CLIO as determined by laser scanning confocal microscopy. (a, e) Area from tumour centre, original magnification 200×. (a) Distribution of Cy5.5-CLIO in Cy5.5 channel; (b) GFP channel; (c) anti-CD11b staining for microglia and macrophages in rhodamine channel; (d) overlay of (a) and (c); (e) overlay of (a) and (b). Cy5.5-CLIO is internalised predominantly by microglial cells and infiltrating macrophages. (f h) Area from tumour–brain interface, tumour border in GFP channel; (g) tumour border in rhodamine channel (CD11b); (h) overlay of (f) and (g). Cells positive for CD11b extend slightly beyond the border of GFP fluorescence. From [63]