Figure 8
a MSC adhesion on various nanostructures and self-assembled monlayer (SAM) on Au or SiO2 surfaces. MSC spreading was characterized by measuring the cell area in actin filament fluorescence images. The surfaces studied are SWCNTs on Au (SWCNT/Au), SWCNTs on cystamine SAM on Au (SWCNT/Cys), SWCNTs on MHA SAM on Au (CNT/MHA), SWCNTs on APTES SAM on SiO2 (CNT/APTES), ZnO nanowires on Au (ZNO/Au), V2O5 nanowires on cysteamine SAM on Au (V2O5/Cys), OTS SAM on SiO2 (OTS/SiO2), and APTES SAM on SiO2 (APTES/SiO2). b Fluorescence microscope image of actin filaments in MSCs adsorbed onto SWCNT patterns on Au surface. SWCNTs were adsorbed onto bare Au with ODT SAM as passivation layer. c Optical microscope image of MSCs adhered ontp mwCNTs/ODT SAM patterns (50 μm wide mwCNT regions and 100 μm wide ODT regions) with ODT passivation after 24 h of cell culture. The mwCNT regions appear as dark areas around the MSCs. d Elongation of MSCs on bulk swCNT substrates or swCNT line patterns as in (b). e Fluorescence microscope image of vinculins represaenting focal adhesions of MSC adsorbed onto swCNT patterns on Au. f Immunofluorescence image of the fibronectins adsorbed on the swCNT patterns on Au substrate [85]