Figure 8

Immunostaining to show organization of actin filament and distribution of vinculin in cells cultured on 10-nm, 50-nm, 100-nm, and 200-nm nanodot arrays and on flat surfaces. Cells were seeded on the arrays for 96 h before harvest. The sample was incubated with anti-vinculin antibody (properly diluted in 0.5% BSA) and phalloidin, followed by incubating with Alexa Fluor 488 goat anti-mouse antibody