Figure 4

Differential ZnO NP cytotoxicity between naive and memory T cells. Human peripheral blood PBMC were left untreated or treated with 10 mM ZnO NPs (8-nm) for 22–24 h and viability determined by monitoring PI uptake using flow cytometry. T cells were defined as CD3⁺, naive T cells as CD3⁺, CD45RA⁺, and memory T cells as CD3⁺, CD45RO⁺. Data from three independent experiments are presented anderror bars depict SE.Asterisks denote statistically significant differences (p < 0.05) between NP treatment groups using as a one-way repeated measures ANOVA